Emerging Pathogens Laboratory

The University of Iowa, College of Public Health

Department of Epidemiology

Human Viral Isolate Reference Sheet

Catalogue Item Name: Ad 22-1

Classification: Adenovirus serotype 22 Strain: undetermined

Passage: 2 Culture Host: A549 cells Time period Host cells infected: 4 days

Original Source Information: This adenovirus isolate was obtained from an Iowa virology laboratory. It was cultured from bronchial specimens collected in March 2002 from a hospitalized 37–year-old male with pneumonia seen in Iowa. The patient died during the hospitalization.

Method of Identification Confirmation: A hospital virology laboratory performed cell culture and adenovirus monoclonal fluorescent antibody staining confirmation. The Emerging Pathogens Laboratory repeated cell culturing on A549 cells, for microneutralization serotyping using antisera 1-5, 7, and 21. Results of the microneutralization testing were uninterpretable. Restriction enzyme analysis, of the purified DNA with BamHi revealed a pattern differing from the Ad 7 strains given to us by CDC (Ad 7a, Ad 7b, Ad 7d/d2, Ad 7h, and Ad 7p). The isolate was given to the CDC for identification who determined it to be an adenovirus of species D and further serotyped it as adenovirus serotype 22.

Reference: M. Noda, T. Yoshida, T. Sakaguchi, Y. Ikeda, K. Yamaoka, and T. Ogino. 2002. Molecular and Epidemiological Analyses of Human Adenovirus Type 7 Strains Isolated from the 1995 Nationwide Outbreak in Japan. J. Clin. Microbiol.

Biosafety Level: 2

Permits/ Forms: A University of Iowa Material Transfer Agreement form must be accepted prior to distributing this viral isolate. The point of contact is:

Dwight Ferguson
Emerging Pathogens Laboratory
University of Iowa
2501 Crosspark Rd
Coralville, IA 52241
ph:  319 335-4983
fax:  319 335-4984
cph-CEIDcatalog@uiowa.edu