Emerging Pathogens Laboratory
The
Department of Epidemiology
Human Viral Isolate Reference Sheet
Catalogue Item Name: Ad 7h-1
Classification: Adenovirus serotype 7 Strain: genotype 7h
Passage: 3 Culture Host: A549 cells Time period Host Cells Infected: 3-5 days
Original Source
Information: This isolate was
collected in December 1999 from the nasal wash of a 1-year old male in an
Method of Identification
Confirmation: A virology laboratory
performed cell culture and monoclonal fluorescent antibody staining
confirmation. The Emerging Pathogens
Laboratory repeated cell culture using A549 cells, and also performed microneutralization serotyping using antisera
1-5, 7, and 21. The isolate was
determined to be serotype 7. Restriction
enzyme analysis of the purified DNA with BamHi
revealed a pattern consistent with genotype 7h. (Patterns were compared to five
prototype adenovirus serotype 7 of genotypes:
7b, 7d2, 7h, 7p, and 7a, obtained from the CDC)
Reference: M. Noda, T.
Yoshida, T. Sakaguchi, Y. Ikeda, K. Yamaoka, and T. Ogino. 2002.
Molecular and Epidemiological Analyses of Human Adenovirus Type 7
Strains Isolated from the 1995 Nationwide Outbreak in
Biosafety Level: 2
Permits/ Forms: A
Dwight Ferguson
Emerging Pathogens Laboratory
University of Iowa
2501 Crosspark Rd
Coralville, IA 52241
ph: 319 335-4983
fax: 319 335-4984
cph-CEIDcatalog@uiowa.edu