Emerging
Pathogens Laboratory
The
Department
of Epidemiology
Human Viral Isolate Reference Sheet
Catalogue Item Name: Ad 14-2
Classification: Adenovirus 14 by hexon and fiber regions.
Passage: 2 Culture Host: A549 cells Time Period Host Cells Infected: 3 days
Original Source Information: This isolate was collected in April 2007 from a stool sample from a 1 year old female seen in Wisconsin.
Method of Identification Confirmation: The sample was typed by sequencing PCR products of partial regions of the hexon and fiber genes and compared to our adenovirus sequence library and NCBI. By fieber gene sequence, this isolates is thought to be representative of the emergent Ad14 strains reported in 2007/8.
References:
Lu X, Erdman DD. Molecular typing of human adenoviruses by PCR and sequencing of a partial region of the hexon gene. Arch Virol 2006 Aug;151(8):1587-602.
Xu W, McDonough MC, Erdman DD. Species-specific identification of human adenoviruses by a multiplex PCR assay. J Clin Microbiol 2000 Nov;38(11):4114-20.
Acute Respiratory Disease Associated with Adenovirus Serotype 14 --- Four States, 2006--2007 MMWR November 16, 2007 / 56(45);1181-1184
Metzgar D, Osuna M, Kajon AE, Hawksworth AW, Irvine M, Russell KL. Abrupt emergence of diverse species B adenoviruses at US military recruit training centers. J Infect Dis. 2007 Nov 15;196(10):1465-73.
Binn LN, Sanchez JL, Gaydos JC. Emergence of adenovirus type 14 in US military recruits--a new challenge. J Infect Dis. 2007;196:1436-7
Louie JK, Kajon AE, Holodniy M, Guardia-LaBar L, Lee B, Petru AM, Hacker JK, Schnurr DP. Severe pneumonia due to adenovirus serotype 14: a new respiratory threat? Clin Infect Dis. 2008;46:421-5.
Biosafety Level: 2
Permits/ Forms: A University of Iowa Material Transfer Agreement form must be accepted prior to distributing this viral isolate. The point of contact is:
Dwight Ferguson
Emerging Pathogens Laboratory
University of Iowa
2501 Crosspark Rd
Coralville, IA 52241
ph: 319 335-4983
fax: 319 335-4984
cph-CEIDcatalog@uiowa.edu