Emerging Pathogens Laboratory

The University of Iowa, College of Public Health

Department of Epidemiology


Human Viral Isolate Reference Sheet

 

Catalogue Item Name:  hMPV-10

 

Isolate Published name: IA4-2002

 

GenBank Accension number: DQ312445

 

Classification: Human metapneumovirus (hMPV)   Strain: G gene, B1  

 

Passage: 1    Culture Host: LLC-MK2 cells   

 

Time Period Host Cells Infected:  7, 13, and 20 days

 

Original Source Information:  This specimen was collected in a clinical virology lab in Iowa in December 2002 from a nasal wash sample of a 6 year-old female.

 

Method of Identification Confirmation:  Before evaluating the nasal wash sample specimen for hMPV, it was found to be negative by routine viral culture for other common respiratory pathogens including: influenza A, influenza B, parainfluenza, adenovirus, and RSV.  The specimen was inoculated onto LLC-MK2 cells and incubated for a total of 20 days.  The supernatant tested positive on days 7, 13, and 20 for hMPV by an RT-PCR procedure targeting F gene (347bp amplicon) and N gene (213bp amplicon) as published in JID 2003:187;785-90 and JCM Jan 2003:Vol.4,No.1;100-5 respectively.  The shell vials supernatant was harvested, aliquotted, and frozen at -70ēC on days 7, 13, and 20.

 

Biosafety Level: 2

 

Permits/ Forms:  A University of Iowa Material Transfer Agreement form must be accepted prior to distributing this specimen.  The point of contact for this agreement is

 

Dwight Ferguson
Emerging Pathogens Laboratory
University of Iowa
2501 Crosspark Rd
Coralville, IA 52241
ph:  319 335-4983
fax:  319 335-4984
cph-CEIDcatalog@uiowa.edu