Emerging
Pathogens Laboratory
The
Department
of Epidemiology
Human Viral Isolate Reference Sheet
Catalogue Item Name: hMPV-11
Isolate Published name: IA5-2002
GenBank Accension number: DQ312446
Classification: Human metapneumovirus
(hMPV)
Strain: G gene, B1
Passage: 1 Culture
Host: LLC-MK2 cells
Time Period Host Cells Infected: 7,
14, and 21 days
Original Source
Information: This specimen was collected
in a clinical virology lab in
Method of Identification
Confirmation: Before evaluating the nasal
wash sample specimen for hMPV, it was found to be negative by routine viral
culture for other common respiratory pathogens including: influenza A,
influenza B, parainfluenza, adenovirus, and RSV. The specimen was inoculated onto LLC-MK2
cells and incubated for a total of 21 days.
CPE was observed on 14 and 21 days.
The supernatant tested positive on days 7, 14, and 21 for hMPV by
an RT-PCR procedure targeting F gene (347bp amplicon) and N gene (213bp amplicon)
as published in JID 2003:187;785-90 and JCM Jan 2003:Vol.4,No.1;100-5
respectively. The shell vials
supernatant was harvested, aliquotted, and frozen at -70ºC on days 7, 14, and 21.
Dr. Boivin’s
laboratory (Université Laval, Quebec City, Canada)
has validated this as a hMPV and typed it as G gene
group 2B as per phylogram published in J General
Virology 2004;85:679-686.
Biosafety Level: 2
Permits/ Forms: A
Dwight Ferguson
Emerging Pathogens Laboratory
University of Iowa
2501 Crosspark Rd
Coralville, IA 52241
ph: 319 335-4983
fax: 319 335-4984
cph-CEIDcatalog@uiowa.edu