Emerging Pathogens Laboratory

The University of Iowa, College of Public Health

Department of Epidemiology

Human Viral Isolate Reference Sheet

Catalogue Item Name: hMPV-4

Isolate Published name: Peru1-2002

GenBank Accension number: DQ393715

Classification: Human metapneumovirus (hMPV) Strain: G gene, B2

Publication: This viral isolate was presented as Peru1-2002 in the phylogram published in Emerg Infect Dis 2006:12:347-350.

Passage: 1 Culture Host: LLC-MK2 cells

Time Period Host cells Infected: 7, 14, 20, and 22 days

Original Source Information: Specimen collected in South America in October 2002 from a 4 year-old patient with a flu-like illness.

Method of Identification Confirmation: Before evaluating the viral throat culture specimen for hMPV, it was found to be negative by routine viral culture for other common respiratory pathogens including: influenza A; influenza B; parainfluenza 1, 2, and 3; adenovirus; and RSV. The specimen was evaluated for hMPV by an RT-PCR procedure targeting the F gene (347bp amplicon) as published in JID 2003:187; 785-90. This testing resulted in a (3+) band and was reproducible upon repeat testing. The specimen was inoculated onto two LLC-MK2 shell vials. The cell culture media was aliquotted and frozen at -70° on days 7, 14, 20, and 23. No CPE was observed until three weeks. Supernatants were positive for hMPV by the above RT-PCR procedure. The 7-day supernatant gave a (2+) RT-PCR bands, the 14, 20, and 22 day supernatant gave (3+) bands. The sample was typed by sequencing PCR products of a partial region of the G gene and compared to our hMPV sequence library and NCBI.

Biosafety Level: 2

Permits/ Forms: A University of Iowa Material Transfer Agreement form must be accepted prior to distributing this viral isolate. The point of contact for this agreement is

Dwight Ferguson
Emerging Pathogens Laboratory
University of Iowa
2501 Crosspark Rd
Coralville, IA 52241
ph:  319 335-4983
fax:  319 335-4984
cph-CEIDcatalog@uiowa.edu