Emerging
Pathogens Laboratory
The
Department
of Epidemiology
Human Viral Isolate Reference Sheet
Catalogue Item Name: Ad 7d2-2
Classification: Adenovirus serotype 7 Strain:
genotype 7d2
Passage: 3 Culture Host: A549 cells Time Period Host Cells
Infected: 3-5 days
Original Source
Information: This isolate was
obtained in February 2001 from the nasal wash of a 10-year-old female seen at a
family medicine clinic in
Method of Identification
Confirmation: A virology laboratory
performed cell culture and monoclonal fluorescent antibody staining
confirmation. The Emerging Pathogens
Laboratory repeated cell culturing using A549 cells, and also performed
microneutralization serotyping using antisera 1-5, 7, and 21. The isolates were determined to be serotype
7. Restriction enzyme analysis, of the
purified DNA with BamHi, revealed a pattern
consistent with genotype 7d2. (Patterns
were compared to five prototype adenovirus serotype 7 of genotypes: 7b, 7d2, 7h, 7p and 7a, obtained from the
CDC)
Reference: M. Noda, T.
Yoshida, T. Sakaguchi, Y. Ikeda, K. Yamaoka, and T. Ogino. 2002.
Molecular and Epidemiological Analyses of Human Adenovirus Type 7
Strains Isolated from the 1995 Nationwide Outbreak in
Biosafety Level: 2
Permits/ Forms: A
Dwight Ferguson
Emerging Pathogens Laboratory
University of Iowa
2501 Crosspark Rd
Coralville, IA 52241
ph: 319 335-4983
fax: 319 335-4984
cph-CEIDcatalog@uiowa.edu