Emerging Pathogens Laboratory

The University of Iowa, College of Public Health

Department of Epidemiology

Human Viral Isolate Reference Sheet

Catalogue Item Name: Ad 7d2-3

Classification: Adenovirus serotype 7 Strain: genotype 7d2

Passage: 3 Culture Host: A549 cells Time Period Host Cells Infected: 3-5 days

Original Source Information: This isolate was collected in February 2001 from the nasal wash of a 1 year old male seen in an Iowa family practice clinic.

Method of Identification Confirmation: A virology laboratory performed cell culture and monoclonal fluorescent antibody staining confirmation. The Emerging Pathogens Laboratory repeated cell culturing using A549 cells, and also performed microneutralization serotyping using antisera 1-5, 7 and 21. The isolates were determined to be serotype 7. Restriction enzyme analysis, of the purified DNA with BamHi, revealed a pattern consistent with genotype 7d2. (Patterns were compared to five prototype adenovirus serotype 7 of genotypes: 7b, 7d2, 7h, 7p, and 7a, obtained from the CDC)

Reference: M. Noda, T. Yoshida, T. Sakaguchi, Y. Ikeda, K. Yamaoka, and T. Ogino. 2002. Molecular and Epidemiological Analyses of Human Adenovirus Type 7 Strains Isolated from the 1995 Nationwide Outbreak in Japan. J. Clin. Microbiol. 40:140-145.

Biosafety Level: 2

Permits/ Forms: A University of Iowa Material Transfer Agreement form must be accepted prior to distributing this viral isolate. The point of contact is:

Dwight Ferguson
Emerging Pathogens Laboratory
University of Iowa
2501 Crosspark Rd
Coralville, IA 52241
ph:  319 335-4983
fax:  319 335-4984
cph-CEIDcatalog@uiowa.edu