Emerging
Pathogens Laboratory
The
Department
of Epidemiology
Human Viral Isolate Reference Sheet
Catalogue Item Name: Ad 7d2-1
Classification: Adenovirus serotype 7 Strain: genotype 7d2
Passage: 3 Culture Host: A549 cells Time Period Host Cells
Infected: 3-5 days
Original Source
Information: This isolate was
collected in February 2001 from the nasal wash
of a 1 year old male seen in an
Method of Identification
Confirmation: The hospital virology
laboratory performed cell culture and monoclonal fluorescent antibody staining
confirmation. The Emerging Pathogens
Laboratory repeated cell culturing using A549 cells, and also performed microneutralization
serotyping using antisera 1-5, 7, and 21.
The isolates were determined to be serotype 7. Restriction enzyme analysis, of the purified
DNA with BamHi, revealed a pattern consistent with genotype 7d2. (Patterns were
compared to five prototype adenovirus serotype 7 of genotypes: 7b, 7d2, 7h, 7p, and 7a, obtained from the
CDC)
Reference: M. Noda, T.
Yoshida, T. Sakaguchi, Y. Ikeda, K. Yamaoka, and T. Ogino. 2002. Molecular and Epidemiological Analyses of
Human Adenovirus Type 7 Strains Isolated from the 1995 Nationwide Outbreak in
Biosafety Level: 2
Permits/ Forms: A
Dwight Ferguson
Emerging Pathogens Laboratory
University of Iowa
2501 Crosspark Rd
Coralville, IA 52241
ph: 319 335-4983
fax: 319 335-4984
cph-CEIDcatalog@uiowa.edu