Emerging Pathogens Laboratory

The University of Iowa, College of Public Health

Department of Epidemiology

Human Viral Isolate Reference Sheet

Catalogue Item Name: hMPV-3

Isolate Published name: Peru2-2002

GenBank Accension number: DQ393716

Classification: Human metapneumovirus (hMPV) Strain: G gene, B1

Publication: This viral isolate was presented as Peru2-2002 in the phylogram published in Emerg Infect Dis 2006:12:347-350.

Passage: 1 Culture Host: LLC-MK2 cells

Time period Host cells infected: 7, 14, 20 and 22 days

Original Source Information: Specimen collected in Cusco, Peru in June 2002 from a male patient aged 4 years and 8 months old with a flu-like illness. Patient was not hospitalized. He had an oral temperature of 38°C. Other symptoms included: malaise, headache, nausea, and cough.

Method of Identification Confirmation: Before evaluating the viral throat culture specimen for hMPV, it was found to be negative by routine viral culture for other common respiratory pathogens including: influenza A; influenza B; parainfluenza 1, 2, and 3; adenovirus; and RSV. The specimen was evaluated for hMPV by an RT-PCR procedure targeting the F gene (347bp amplicon) as published in JID 2003:187; 785-90. This testing resulted in a (3+) band, which was reproducible upon repeat testing. The specimen was inoculated onto LLC-MK2 cells. The cell culture media was aliquotted and frozen at -70° on days 7, 14, 20, and 23. No CPE was observed until three weeks. Supernatants were positive for hMPV by the above RT-PCR procedure. The 7-day supernatant gave a (1+) RT-PCR band, the 14-day gave a (2+) band and the 20-day gave (3+) band. The specimen was originally set up on 3 shell-vials, of which 2 became contaminated with yeast. The following day the specimen was filtered (0.45um) and inoculated onto 3 additional shell-vials held 18 days. Supernatants from these vials were collected on days 7, 15 and 18 and gave (1+) RT-PCR bands with the previously described procedure, suggesting filtering of the specimen may have lead to a decrease in positivity. Dr. Yacine Abed (Université Laval, Quebec City, Canada) has typed the isolate as a G gene group B1 per Emerg Infect Dis 2005:11:1074-78.

Biosafety Level: 2

Permits/ Forms: A University of Iowa Material Transfer Agreement form must be accepted prior to distributing this viral isolate. The point of contact is:

Dwight Ferguson
Emerging Pathogens Laboratory
University of Iowa
2501 Crosspark Rd
Coralville, IA 52241
ph:  319 335-4983
fax:  319 335-4984
cph-CEIDcatalog@uiowa.edu