Emerging Pathogens Laboratory

The University of Iowa, College of Public Health

Department of Epidemiology

Human Viral Isolate Reference Sheet

Catalogue Item Name: hMPV-9

Isolate Published name: IA3-2002

GenBank Accension number: DQ312444

Classification: Human metapneumovirus (hMPV) Strain: G gene, A1

Passage: 1 and 2 Culture Host: LLC-MK2 cells

Time Period Host Cells Infected: Days 7, 13, & 20 for P1. Days 6, 13, & 20 for P2.

Original Source Information: This specimen was collected in a clinical virology lab in Iowa in March 2002 from a nasal wash sample of a 1 year-old male.

Method of Identification Confirmation: Before evaluating the nasal wash sample specimen for hMPV, it was found to be negative by routine viral culture for other common respiratory pathogens including: influenza A, influenza B, parainfluenza, adenovirus, and RSV. The specimen was inoculated onto LLC-MK2 cells and incubated for a total of 20 days. CPE was observed at 20 days for passage 1. The supernatant tested positive on days 6, 7, 13, and 20 for hMPV by an RT-PCR procedure targeting F gene (347bp amplicon) and N gene (213bp amplicon) as published in JID 2003:187;785-90 and JCM Jan 2003:Vol.4,No.1;100-5 respectively. The shell vials supernatant was harvested, aliquotted, and frozen at -70ºC on days 7, 13, and 20 for passage 1 and 6, 13, and 20 for passage 2. Dr. Boivin’s laboratory (Université Laval, Quebec City, Canada) has validated this as a hMPV and typed it as G gene group 1B as per phylogram published in J General Virology 2004;85:679-686.

Biosafety Level: 2

Permits/ Forms: A University of Iowa Material Transfer Agreement form must be accepted prior to distributing this specimen. The point of contact for this agreement is

Dwight Ferguson
Emerging Pathogens Laboratory
University of Iowa
2501 Crosspark Rd
Coralville, IA 52241
ph:  319 335-4983
fax:  319 335-4984
cph-CEIDcatalog@uiowa.edu